Neofect™ DNA transfection reagent

Neofect™ DNA transfec on reagent 非脂质阳离子聚合物为主的转染试剂,是新一代非病毒转染试剂。
可以与 DNA 形成稳定的复合物,透过细胞膜进入细胞内,并保护 DNA 免受核酸酶的降解。该试剂对细胞毒性


  • 低细胞毒性—更温和
  • 高转染效率—更高效
  • 性价比更高—更经济
  • 操作更方便—更简单


Neofect™ DNA transfection reagent是一种非常温和的转染试剂,相比脂质体转染试剂,表现出更低的细胞毒性。在10倍使用浓度下,仍无明显细胞毒性.



Neofect™ DNA transfection reagent转染效率与优势脂质体转染试剂LP2K相比,表现出更高的转染效率。


使用Neofect™ DNA transfection reagent转染细胞,非常方便只要简单的一比一等量混合
(1ug DNA :1ul Neofect™),即可实现完美转染。相对与其它转染试剂1:2或1:4的使用浓度,转染试剂的使用量更少,价格更优惠。同时更少的DNA的质量,对细胞伤害更小。

Reagent Culture vessel Volume of reagent Amount of DNA
Neofect™ 6-well/35 mm dish 2μL 2μg
L2K 6-well/35 mm dish 10μL 4 µg


使用Neofect DNA transfection reagent 只需要简单的三步即可完成转染实验,一般情况下转染后无需换液,即可实现高效转染。



货号 产品名 规格 单价
TF201201(TF20121201) Neofect™ DNA transfection reagent 1mL 1500


  • [1]. Sun L, Zhou R, Yang G, et al. Analysis of 138 pathogenic mutations in presenilin-1 on the in vitro production of Aβ42 and Aβ40 peptides by γ-secretase[J]. Proceedings of the National Academy of Sciences, 2016: 201618657.
  • [2]. Zhao X Q, Zhu L L, Chang Q, et al. C-type lectin receptor dectin-3 mediates trehalose 6, 6′-dimycolate (TDM)-induced Mincle expression through CARD9/Bcl10/MALT1-dependent nuclear factor (NF)-κB activation[J]. Journal of Biological Chemistry, 2014, 289(43): 30052-30062.
  • [3]. Gao X, Shi X, Fu X, et al. Human Tudor staphylococcal nuclease (Tudor‐SN) protein modulates the kinetics of AGTR1‐3′ UTR granule formation[J]. FEBS letters, 2014, 588(13): 2154-2161.
  • [4]. Luo Q, Liu X, Zheng Y, et al. Ephrin-B2 mediates trophoblast-dependent maternal spiral artery remodeling in first trimester[J]. Placenta, 2015, 36(5): 567-574.
  • [5]. Hao R, He J, Liu X, et al. Inhibition of hepatitis B virus gene expression and replication by hepatocyte nuclear factor 6[J]. Journal of virology, 2015, 89(8): 4345-4355.
  • [6]. Liu X, Hu Y, Liu X, et al. EPHB4, a down stream target of IFN‐γ/STAT1 signal pathway, regulates endothelial activation possibly contributing to the development of preeclampsia[J]. American Journal of Reproductive Immunology, 2016, 76(4): 307-317.
  • [7]. Li R, Li Y, Hu X, et al. Transcription factor 3 controls cell proliferation and migration in glioblastoma multiforme cell lines[J]. Biochemistry and Cell Biology, 2016, 94(3): 247-255.
  • [8]. Liu X, Hu Y, Zheng Y, et al. EPHB4 Regulates Human Trophoblast Cell Line HTR-8/SVneo Function: Implications for the Role of EPHB4 in Preeclampsia 1[J]. Biology of Reproduction, 2016, 95(3): 65, 1-11.
  • [9]. Wang L, Xu K, Ren C, et al. Targeted Deletion of Vitamin D receptor Gene in Mammalian Cells by CRISPR/Cas9 Systems[J]. bioRxiv, 2016: 040147.
  • [10]. Li P, Sun X, Ma Z, et al. Transcriptional Reactivation of OTX2, RX1 and SIX3 during Reprogramming Contributes to the Generation of RPE Cells from Human iPSCs[J]. International journal of biological sciences, 2016, 12(5): 505.
  • [11]. Liu X, Luo Q, Zheng Y, et al. NOTCH4 signaling controls EFNB2-induced endothelial progenitor cell dysfunction in preeclampsia[J]. Reproduction, 2016, 152(1): 47-55.
  • [12]. Zhang T, Yin Y, Liu H, et al. Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System[J]. PloS one, 2016, 11(9): e0163551.
  • [13]. Geng T, Huang Y, Hou C, et al. Inductive expression patterns of genes related to Toll signaling pathway in silkworm (Bombyx mori) upon Beauveria bassiana infection[J]. Journal of Asia-Pacific Entomology, 2016, 19(3): 861-868.
  • [14]. Xiong Y, Liu Y, Xiong W, et al. Hypoxia-inducible factor 1α-induced epithelial–mesenchymal transition of endometrial epithelial cells may contribute to the development of endometriosis[J]. Human Reproduction, 2016, 31(6): 1327-1338.
  • [15]. Liu H, Ren C, Zhu B, et al. High-Efficient Transfection of Human Embryonic Stem Cells by Single-Cell Plating and Starvation[J]. Stem cells and development, 2016, 25(6): 477-491.
  • [16]. Wang H, Zhang C, Xu L, et al. Bufalin suppresses hepatocellular carcinoma invasion and metastasis by targeting HIF-1α via the PI3K/AKT/mTOR pathway[J]. Oncotarget, 2016, 7(15): 20193-20208.
  • [17]. Wang Y, Liang D, Zhu Z, et al. Bone morphogenetic protein-7 prevented epithelial-mesenchymal transition in RLE-6TN cells[J]. Toxicology Research, 2016, 5(3): 931-937.
  • [18]. Geng T, Lv D D, Huang Y X, et al. JAK/STAT signaling pathway-mediated immune response in silkworm (Bombyx mori) challenged by Beauveria bassiana[J]. Gene, 2016, 595(1): 69-76.
  • [19]. Liu W, Liu X, Luo M, et al. dNK derived IFN-γ mediates VSMC migration and apoptosis via the induction of LncRNA MEG3: A role in uterovascular transformation[J]. Placenta, 2017, 50: 32-39.
  • [20]. Liu X, Luo Q, Zheng Y, et al. Notch1 Impairs Endothelial Progenitor Cell Bioactivity in Preeclampsia[J]. Reproductive Sciences, 2017, 24(1): 47-56.